A novel fluorometric assay method of dopamine-.BETA.-hydroxylase activity in human serum.
نویسندگان
چکیده
منابع مشابه
Serum dopamine-beta-hydroxylase activity.
Dopamine-yS-liydroxylase, the enzyme which converts dopamine to norepinephrine, is released into the perfusate upon stimulation of the isolated perfused adrenal gland and after stimulation of the nerves to the isolated perfused spleen. This study was undertaken to determine whether dopamine-/?hydroxylase activity could be detected circulating in blood. By using a sensitive new enzymatic assay, ...
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In the present study, an oxidative conversion of non-fluorescent dichlorofluoroscein (LDCF) to fluorescent dichlorofluoroscein (DCF) was used for detection of antioxidant properties of plant extracts. The rate of the reaction was followed by monitoring the formation of DCF as a function of time. The antioxidant assay was carried out for different concentrations of gallic acid, Salvia ...
متن کاملSimple photometric assay of dopamine-beta-hydroxylase activity in human blood: useful in clinical chemistry.
Featured Article: Nagatsu T and Udenfriend S. Photometric assay of dopamine-hydroxylase in human blood. Clin Chem 1972;18:980 –3. This report described a simple assay for measuring the activity of dopamine-hydroxylase (DBH) in human blood. DBH is a copper-containing, ascorbaterequiring glycoprotein enzyme that catalyzes the hydroxylation of dopamine to norepinephrine (NE), the precursor of epin...
متن کامل[Experimental and clinical studies on the activity of human serum dopamine-beta-hydroxylase].
Dopamine-(9-Hydroxylase is an enzyme which catalyzes dopamine to noradrenaline in final step of catecohlamine synthesis. This study was undertaken to determine the human serum Dopaminelj3-Hydroxylase activity and examine effects of fusaric acid (5-butylpicolinic acid) on this enzyme activity in vitro and in vivo. A sensitive method for measurement of the human serum Dopamine-8-Hydroxylase activ...
متن کاملA study of the possible relationship of serum dopamine-beta-hydroxylase activity with diabetic neuropathy.
Serum DBH activity in a normal population of 58 individuais shows a gaussian distribution, on the opposite to what happens in a popuiation of 100 diabetics. These two populations also differ statistically by the analysis of variance. However it was round that it is not possible to correiate ...rum DBH neither with insulin admi nistration, type of diabetes, duration of disease, nor with the seve...
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ژورنال
عنوان ژورنال: Chemical and Pharmaceutical Bulletin
سال: 1977
ISSN: 0009-2363,1347-5223
DOI: 10.1248/cpb.25.748